Peptide Synthesis

Service description

Visit website https://www.cib.csic.es/facilities/scientific-facilities/protein-chemistry Peptide synthesizer (AAPPtec, Focus XC). It uses Fmoc chemistry, and it can simultaneously synthesize up to six peptides in a range of 0.05-5 mmol. Synthesis is carried out at 100 µmole, 250 µmole or 1 nmole scales. On an average, a synthesis scale of 100 µmoles will yield about 50-70 mg of a 10-residue peptide and 100-150 mg of a 20-residue peptide depending on the substitution level of the resin. After the synthesis, the peptide is de-protected and cleaved from the resin with trifluoroacetic acid, precipitated with ether and lyophilized. The lyophilized material will be provided as a "crude" preparation. Turnaround time is about ten days to three weeks. The core has a strict quality control in peptide synthesis. An LC-MS analysis will be provided for every peptide as assessment of the quality of synthesized product. When necessary MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time Of Flight) mass spectrometry for peptide can be requested. The laboratory can also provide the amino acid analysis of the syntezised peptide if requested. The peptides are routinely synthesized as peptides amides or N-acetylated derivatives. Peptides containing another unusual or modified amino acids (e.g., phospho-Tyr, phospho-Ser/Thr, biotinylation, fluorochrome conjugation (fluorescein, rhodamine) can be synthesized but the requester will be charged the additional costs required to carry out this synthesis.