Affinity chromatography: Enrichment in phosphopeptides for proteomics

Service description

For phosphopeptide enrichment we use The Thermo Scientific High-Select Fe-NTA Phosphopeptide Enrichment Kit (A32992) and The High-Select TiO2 Phosphopeptide Enrichment Kit (A32993). Both of them enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). The High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Recovers peptides with multiple phosphorylation sites preferentially to monophosphorylated ones. The High-Select¿ TiO2 Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using TiO2 resin in spin columns. Recovers monophosphorylated peptides preferentially. The TiO2 Kit is complementary to the Fe-NTA Kit. Each column can enrich phosphopeptides from 0.5 to 3-5 mg of total protein digest as starting sample. Use of these kits results in enrichment of up to 150 µg of phosphopeptides with greater than 90% selectivity.