Protein Separation by HPLC

Service description

Protein chromatographic separations are performed using an FPLC ÄKTApurifier (GE Healthcare). ÄKTApurifier is a complete fully automated liquid chromatography system designed for research applications. It can work at flow rates up to 10 ml / min and pressures up to 25 MPa. It is equipped with an UV detector and a fraction collector, among other components. The different chromatographies can be performed at the analytical scale or at the semi-preparative scale (in which the separation of proteins and collection of the different fractions is carried out). The user will provide the necessary buffers or solvents to perform the chromatography. The following columns (GE Healthcare) are available: - Superdex 75 10/300 GL - Superdex 200 10/300 GL - URPC C2 / C18 ST - Mini S PE - Source 15 RPC - Resource Q - Mono Q HR 5/5 - Mini Q PE - Mono S HR 5/5 - XK 26/20 Q Sepharose - SP Sepharose XK 26/20 SAMPLES SUBSCRIPTION INSTRUCTIONS The samples will be delivered together with the corresponding application properly filled out ("LIQUID CHROMATOGRAPHY REQUEST), that can be found at website http://www.ipb.csic.es/servicios/Proteomica/formularios%202.0.html) and then retourned to the Proteomic Unit. Optionally, the request form can be emailed to: "proteomica@ipb.csic.es". The user must previously consult the availability of columns and request the completion of chromatography with some time in advance (7-15 days).