Bio-Layer Interferometry | Consejo Superior de Investigaciones Científicas

Service data

Support unit: Interacciones Moleculares
Institute: CENTRO DE INVESTIGACIONES BIOLOGICAS MARGARITA SALAS
Locality: Madrid (Madrid)
Service's web: https://www.cib.csic.es/es/servicios/servicios-cientificos/interacciones-moleculares

Service's description

El Servicio de Interacciones Moleculares se encarga del estudio cuantitativo (tamaño, masa molecular, estado de asociación/estequiometría y afinidad) de macromoléculas y complejos macromoleculares en disolución, del tipo proteína-proteína, proteína-ADN y proteína-ligando, además de prestar asesoramiento y apoyo al usuario en la ejecución de técnicas biofísicas de dispersión de luz dinámica (DLS) y estática multiángulo (SEC-MALS), interferometría de biocapa (BLI), medidas de intensidad y anisotropía de fluorescencia y espectroscopía de correlación de fluorescencia (FCS). En 2009 el laboratorio consiguió la certificación conforme a la norma ISO9001 con el código ER-0286/2009.

Benefit description

The BLItz system enables real-time quantitation of solution-based analytes or kinetic characterization of molecular interactions. It is a label-free direct binding assay which takes place on a disposable biosensor made from a biocompatible matrix that is uniform, non-denaturing and minimizes non-specific binding. Only molecules that bind directly to the biosensor surface are detected, providing exceptional specificity for individual applications, even in crude media. A variety of disposable, off-the-shelf biosensors are available, making it easy to run a wide range of assays on the same system. The BLItz system emits white light down the biosensor, and then collects any light reflected back. Reflected wavelengths are affected by the thickness of the coating on the optical layer. Some wavelengths show constructive interference, others show destructive interference. This interference is captured by a spectrometer as a unique spectral signature and is reported in relative intensity units (nm). Any change in the number of molecules bound to the biosensor causes a shift in the interference pattern that is measured in real time. This wavelength shift is a direct measure of the change in optical thickness (nm) of the biological layer.

Options and prices chart
Options	Unit	Public Sector	Other customers
General	€/hora/biosensor	32.43 €	35.49 €

Benefit description

Biolayer interferometry (BLI) is performed with the 8-channel Octet RED 96 (Sartorius). It allows for real-time, label-free kinetic analysis and quantification of molecular affinity interactions. The system offers a wide variety of biosensors. The user laboratory can request the analysis plates from the Unit or provide them from its inventory. The biosensors to be used will be purchased directly from the supplier.

Options and prices chart
Options	Unit	Public Sector	Other customers
Octet - Servicio	€ / dia	116.63 €	127.74 €
Placa 96	€/ placa 96	12.88 €	14.1 €

Support unit	Support unit Information	Selected service	Other services
Interacciones Moleculares CIB - Madrid
UNIDAD DE PROTEÓMICA IPBLN - Armilla