Malondialdehyde Determination

Benefit description

Quantification of MDA is an indicator of lipid peroxidation due to oxidative stress in the fruit cells. Oxidative stress occurs when the generation of ROS exceeds the capacity of plant to maintain cellular redox homeostasis or to scavenge ROS. ROS accumulation may cause oxidative damage to lipids, forming toxic products such as malondialdehyde (MDA). The increase of MDA level indicates a distinct deterioration of membrane integrity and activation of lipid peroxidation in the fruit. Its quantification is carried out by means of a biochemical reaction with MDA whose product absorbs at a certain wavelength.