Descripción del servicio
Description:
DNA fragmentation is a key step for genomic library construction and its subsequent application to high throughput sequencing protocols, among other applications (see details on the applications section). Among the different methods available (physical, chemical and enzymatic shearing), our service offers fragmentation by sonication, a process which consists of hydrodynamic shearing of DNA samples through short periods of controlled sonication. This process is highly efficient in comparison to other methods, reaching high levels of sensitivity. It is reproducible and not influenced by technical manipulation, laboratory conditions and change of equipment used.
Equipment available:
Qsonica Sonicator Q800R3 (https://www.sonicator.com/products/q800r-sonicator). DNA and chromatin shearing system in liquid. This equipment allows fragmentation of DNA within a range of sizes considerably wide (from 150 bp to 3 Kb). Fragment size needs to be optimized and will depend on the methodological goal. The system allows simultaneous multiplexing of up to 18 samples. The volumen required per sample ranges between 50 µl and 1 mL. Standard comercial tube sizes for molecular lab experiments are used (0.3 mL PCR tubes, 0.5 mL PCR PCR tubes and 1,5 mL poliestirene Eppendorf tubes).
Catalogue of technical applications:
-Production of DNA fragments for genomic library construction of application for high throughput sequencing (NGS), including: : whole genome sequencing, genome skimming, sequence capture, RAD-seq, GBS.
- Chromatin fragmentation
- ChIP and ChIP-seq
- Transcriptomics (RNA-seq)
- Protein extraction
- Cell lysis
Service offered:
- Sample preparation advice and simple analysis
- Preliminary adjustment test for sonication time and experimental adjustments
- Protocol optimization and laboratory technical assistance. Troubleshooting where required
Cuadro de opciones y precios
| Opciones |
Unidad |
Sector Público |
Otros Clientes |
| Fragmentación de ADN (10 muestras) |
10 muestras |
56.18 € |
61.53 € |