Desalting and concentration by Zip-Tip

Service description

Desalting of the peptides obtained after enzymatic digestion of proteomes using C18 resin: Zip-tip (Millipore), OMIX Pipette tips (Agilent Technologies). The ZipTip is a 10 uL (P-10) pipette tip with a bed of chromatography media fixed at its end such that there is no dead volume. It is intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. The sample is aspirated and dispensed through ZipTip to bind, wash, and elute. Recovered samples are contaminant-free and eluted in 0.5-4 uL for direct transfer to a mass spectrometer target or vial. The OMIX tip is conditioned with wetting and equilibration solutions to ensure optimum binding. During sample application, the peptides and proteins have a strong affinity for the Bond Elut OMIX hydrophobic monolithic silica surface. Salts, detergents and other hydrophilic contaminants present from proteolytic digests pass through the tip unretained. Residual contaminants that are weakly bound to the Bond Elut OMIX sorbent material are washed from the tip with a slightly acidic water rinse. The target peptides are recovered in their concentrated and purified form with a slightly acidic, aqueous-organic solvent.